From structure-based predictions, to exploring cellular function

 

A new structure carries a wealth of information and raises numerous hypotheses that require experimental validation.  Using the latest biochemical and biophysical techniques, we can probe how our new structural insights relate to protein activity, and how ensembles of proteins might work together to drive an important biological process.  Ultimately, we aim to place our findings back in a cellular context, and use bacterial genetics and cell culture models of infection to explore how precise changes in protein sequence and structure impact mutant phenotypes.